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过表达慢病毒 干扰慢病毒
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过表达细胞系构建服务 敲低细胞系构建服务 敲除细胞系构建服务 报告基因细胞株构建服务 Luciferase标记细胞株
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Current position：Technical Service > Lentivirus > 过表达慢病毒 > 案例展示
案例展示
服务简介

将慢病毒载体的过表达质粒，与慢病毒包装辅助质粒和包膜质粒共转染293T细胞，收集上清进行浓缩纯化，获取高滴度的慢病毒，可用于直接感染目的细胞，获得过表达目的基因的细胞系，以用于基因功能研究。

案例展示
使用吉满生物提供的ALKBH5过表达慢病毒感 染BxPC-3细胞，经RT-qPCR和western Blot验 证：感染ALKBH5慢病毒后，ALKBH5的表达 量显著上调；（Mol Cancer. 2020 May 19;19 (1):91.）
使用吉满生物提供STAT3过表达慢病毒感染 MLE12细胞，并进行OGD/R诱导，发现STAT3过 表达后，SLC7A11和pSTAT3的表达量显著增加； （Oxid Med Cell Longev. 2020 Sep 18；2020: 5146982. ）
客户文章节选
IF=41.444,Molecular Cancer,南昌大学第二附属医院,circRNA过表达慢病毒、circRNA干扰慢病毒，cas9敲除细胞系.
The circular RNA circHMGB2 drives immunosuppression and anti-PD-1 resistance in lung adenocarcinomas and squamous cell carcinomas via the miR-181a-5p/CARM1 axis
IF=23.168，Journal of Hematology & Oncology，复旦大学中山医院肝癌研究所，过表达慢病毒
Coexpression of gene Oct4 and Nanog initiates stem cell characteristics in hepatocellular carcinoma and promotes epithelial-mesenchymal transition through activation of Stat3/Snail signaling
IF=17.5213,Advanced Science,山东大学齐鲁医院,过表达慢病毒.
The E3 Ligase TRIM4 Facilitates SET Ubiquitin-Mediated Degradation to Enhance ER-α Action in Breast Cancer
IF=17.521，Advanced Science，上海市新药设计重点实验室，过表达慢病毒
Multi‐Arm PEG/Peptidomimetic Conjugate Inhibitors of DR6/APP Interaction Block Hematogenous Tumor Cell Extravasation
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相关资料
慢病毒操作手册
Current position：Technical Service > Lentivirus > 过表达慢病毒 > 案例展示
classify
案例展示
服务简介

将慢病毒载体的过表达质粒，与慢病毒包装辅助质粒和包膜质粒共转染293T细胞，收集上清进行浓缩纯化，获取高滴度的慢病毒，可用于直接感染目的细胞，获得过表达目的基因的细胞系，以用于基因功能研究。

案例展示
使用吉满生物提供的ALKBH5过表达慢病毒感 染BxPC-3细胞，经RT-qPCR和western Blot验 证：感染ALKBH5慢病毒后，ALKBH5的表达 量显著上调；（Mol Cancer. 2020 May 19;19 (1):91.）
使用吉满生物提供STAT3过表达慢病毒感染 MLE12细胞，并进行OGD/R诱导，发现STAT3过 表达后，SLC7A11和pSTAT3的表达量显著增加； （Oxid Med Cell Longev. 2020 Sep 18；2020: 5146982. ）
客户文章节选
IF=41.444,Molecular Cancer,南昌大学第二附属医院,circRNA过表达慢病毒、circRNA干扰慢病毒，cas9敲除细胞系.
The circular RNA circHMGB2 drives immunosuppression and anti-PD-1 resistance in lung adenocarcinomas and squamous cell carcinomas via the miR-181a-5p/CARM1 axis
IF=23.168，Journal of Hematology & Oncology，复旦大学中山医院肝癌研究所，过表达慢病毒
Coexpression of gene Oct4 and Nanog initiates stem cell characteristics in hepatocellular carcinoma and promotes epithelial-mesenchymal transition through activation of Stat3/Snail signaling
IF=17.5213,Advanced Science,山东大学齐鲁医院,过表达慢病毒.
The E3 Ligase TRIM4 Facilitates SET Ubiquitin-Mediated Degradation to Enhance ER-α Action in Breast Cancer
IF=17.521，Advanced Science，上海市新药设计重点实验室，过表达慢病毒
Multi‐Arm PEG/Peptidomimetic Conjugate Inhibitors of DR6/APP Interaction Block Hematogenous Tumor Cell Extravasation
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相关资料
慢病毒操作手册
Lentivirus
过表达慢病毒 干扰慢病毒
Cell Line Development
过表达细胞系构建服务 敲低细胞系构建服务 敲除细胞系构建服务 报告基因细胞株构建服务 Luciferase标记细胞株
Protein Expression
Plasmid DNA Preparation Services
过表达质粒构建 干扰质粒构建 突变/截短质粒构建 荧光素酶报告基因质粒构建
Adeno-associated Virus
过表达AAV 干扰AAV
Cell-based Assay
抗体Binding检测 蛋白/抗体药物功能检测 ADCC/P 活性检测 ADC药物CTG杀伤检测 CCK8细胞增值检测 克隆形成 细胞迁移/侵袭 细胞周期/凋亡 划痕实验
Antibody Services
Reporter Gene Detection Services
miRNA与靶基因调控研究 转录因子调控/promoter活性研究
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