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过表达慢病毒 干扰慢病毒
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Current position：Technical Service > Lentivirus > 干扰慢病毒 > 案例展示
案例展示
服务简介

将慢病毒载体的shRNA与慢病毒包装辅助质粒和包膜质粒共转染293T细胞，收集上清进行浓缩纯化，获取高滴度的慢病毒，相对于siRNA和shRNA质粒，shRNA慢病毒的感染范围更广，如依赖转染试剂难转染的细胞：原代细胞、悬浮细胞等，并可实现稳定、长效抑制基因表达。

案例展示
使用吉满生物shRNA慢病毒感染NOZ细胞，观察 NOZ细胞的感染效率，并分别感染NOZ细胞和 OCUG细胞，经RT-qPCR和western Blot验证： 感染shRNA慢病毒后，ERR的表达量显著降低。 （Cancer Sci. 2020 May;111(5):1514-1527.）
使用吉满生物提供sh-TRIM44和TRIM44过表达 慢病毒分别感染A2058和A375细胞，经RT-qPCR 和western Blot验证：sh-TRIM44感染后，目的 基因表达量显著降低；感染TRIM44过表达慢病 毒后，目的基因的表达量显著上调； （Cancer Med. 2018 Mar;7(3):796-808.）
客户文章节选
IF=50.3006,Cancer Cell，复旦大学附属中山医院,CRISPR-Cas9敲除细胞系;过表达和干扰慢病毒.
Integrated multi-omics profiling to dissect the spatiotemporal evolution of metastatic hepatocellular carcinoma
IF=41.444，Molecular Cancer，中山医院癌变重点实验室，干扰慢病毒
Circular RNA circMET drives immunosuppression and anti-PD1 therapy resistance in hepatocellular carcinoma via the miR-30-5p/snail/DPP4 axis
IF=41.444,Molecular Cancer,南昌大学第二附属医院,circRNA过表达慢病毒、circRNA干扰慢病毒，cas9敲除细胞系.
The circular RNA circHMGB2 drives immunosuppression and anti-PD-1 resistance in lung adenocarcinomas and squamous cell carcinomas via the miR-181a-5p/CARM1 axis
IF=23.168，Journal of Hematology & Oncology，中山医院，过表达慢病毒、干扰慢病毒、siRNA合成
Downregulation of RNF128 activates Wnt/ β-catenin signaling to induce cellular EMT and stemness via CD44 and CTTN ubiquitination in melanoma
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慢病毒操作手册
Current position：Technical Service > Lentivirus > 干扰慢病毒 > 案例展示
classify
案例展示
服务简介

将慢病毒载体的shRNA与慢病毒包装辅助质粒和包膜质粒共转染293T细胞，收集上清进行浓缩纯化，获取高滴度的慢病毒，相对于siRNA和shRNA质粒，shRNA慢病毒的感染范围更广，如依赖转染试剂难转染的细胞：原代细胞、悬浮细胞等，并可实现稳定、长效抑制基因表达。

案例展示
使用吉满生物shRNA慢病毒感染NOZ细胞，观察 NOZ细胞的感染效率，并分别感染NOZ细胞和 OCUG细胞，经RT-qPCR和western Blot验证： 感染shRNA慢病毒后，ERR的表达量显著降低。 （Cancer Sci. 2020 May;111(5):1514-1527.）
使用吉满生物提供sh-TRIM44和TRIM44过表达 慢病毒分别感染A2058和A375细胞，经RT-qPCR 和western Blot验证：sh-TRIM44感染后，目的 基因表达量显著降低；感染TRIM44过表达慢病 毒后，目的基因的表达量显著上调； （Cancer Med. 2018 Mar;7(3):796-808.）
客户文章节选
IF=50.3006,Cancer Cell，复旦大学附属中山医院,CRISPR-Cas9敲除细胞系;过表达和干扰慢病毒.
Integrated multi-omics profiling to dissect the spatiotemporal evolution of metastatic hepatocellular carcinoma
IF=41.444，Molecular Cancer，中山医院癌变重点实验室，干扰慢病毒
Circular RNA circMET drives immunosuppression and anti-PD1 therapy resistance in hepatocellular carcinoma via the miR-30-5p/snail/DPP4 axis
IF=41.444,Molecular Cancer,南昌大学第二附属医院,circRNA过表达慢病毒、circRNA干扰慢病毒，cas9敲除细胞系.
The circular RNA circHMGB2 drives immunosuppression and anti-PD-1 resistance in lung adenocarcinomas and squamous cell carcinomas via the miR-181a-5p/CARM1 axis
IF=23.168，Journal of Hematology & Oncology，中山医院，过表达慢病毒、干扰慢病毒、siRNA合成
Downregulation of RNF128 activates Wnt/ β-catenin signaling to induce cellular EMT and stemness via CD44 and CTTN ubiquitination in melanoma
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相关资料
慢病毒操作手册
Lentivirus
过表达慢病毒 干扰慢病毒
Cell Line Development
过表达细胞系构建服务 敲低细胞系构建服务 敲除细胞系构建服务 报告基因细胞株构建服务 Luciferase标记细胞株
Protein Expression
Plasmid DNA Preparation Services
过表达质粒构建 干扰质粒构建 突变/截短质粒构建 荧光素酶报告基因质粒构建
Adeno-associated Virus
过表达AAV 干扰AAV
Cell-based Assay
抗体Binding检测 蛋白/抗体药物功能检测 ADCC/P 活性检测 ADC药物CTG杀伤检测 CCK8细胞增值检测 克隆形成 细胞迁移/侵袭 细胞周期/凋亡 划痕实验
Antibody Services
Reporter Gene Detection Services
miRNA与靶基因调控研究 转录因子调控/promoter活性研究
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