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Current position：Technical Service > Reporter Gene Detection Services > 转录因子调控/promoter活性研究 > 案例展示
案例展示
服务简介

转录因子与启动子研究主要包含：

（1）启动子活性验证：验证转录因子与启动子是否存在相互作用；

（2）转录因子与启动子作用位点研究：通过截短、潜在结合位点突变验证转录因子与启动子的结合位点。

案例展示
该研究使用吉满生物提供的pGL3‐Basic载体成功构建Nectin‐4启动子区（0 ~ -2000bp处）野生型和突变型报告基因质粒。在293T细胞中，过表达转录因子ERRα增强了pGL3‐Basic‐Nectin4‐WT‐luc报告基因表达载体的荧光素酶活性（P<0.01)，该结果表明转录因子ERRα促进Nectin‐4基因启动子区转录活性。
客户文章节选
IF=15.1004,Advanced Science，浙江大学医学院附属第二医院,报告基因质粒；启动子与转录因子.
Radiotherapy-Induced Astrocyte Senescence Promotes an Immunosuppressive Microenvironment in Glioblastoma to Facilitate Tumor Regrowth
IF=14.903,Acta Pharmaceutica Sinica B，中国药科大学药学院,质粒，干扰AAV，启动子报告基因质粒.
PXR activation impairs hepatic glucose metabolism partly via inhibiting the HNF4α–GLUT2 pathway
IF=14.5,Brain，同济大学医学院附属上海东方医院,过表达质粒；启动子；报告基因质粒.
Maf1 loss regulates spinogenesis and attenuates cognitive impairment in Alzheimer’s disease
IF=10.334，Pharmacological Research，上海交通大学附属第六医院，启动子报告基因质粒、过表达慢病毒
Imperatorin alleviates cancer cachexia and prevents muscle wasting via directly inhibiting STAT3
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Current position：Technical Service > Reporter Gene Detection Services > 转录因子调控/promoter活性研究 > 案例展示
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案例展示
服务简介

转录因子与启动子研究主要包含：

（1）启动子活性验证：验证转录因子与启动子是否存在相互作用；

（2）转录因子与启动子作用位点研究：通过截短、潜在结合位点突变验证转录因子与启动子的结合位点。

案例展示
该研究使用吉满生物提供的pGL3‐Basic载体成功构建Nectin‐4启动子区（0 ~ -2000bp处）野生型和突变型报告基因质粒。在293T细胞中，过表达转录因子ERRα增强了pGL3‐Basic‐Nectin4‐WT‐luc报告基因表达载体的荧光素酶活性（P<0.01)，该结果表明转录因子ERRα促进Nectin‐4基因启动子区转录活性。
客户文章节选
IF=15.1004,Advanced Science，浙江大学医学院附属第二医院,报告基因质粒；启动子与转录因子.
Radiotherapy-Induced Astrocyte Senescence Promotes an Immunosuppressive Microenvironment in Glioblastoma to Facilitate Tumor Regrowth
IF=14.903,Acta Pharmaceutica Sinica B，中国药科大学药学院,质粒，干扰AAV，启动子报告基因质粒.
PXR activation impairs hepatic glucose metabolism partly via inhibiting the HNF4α–GLUT2 pathway
IF=14.5,Brain，同济大学医学院附属上海东方医院,过表达质粒；启动子；报告基因质粒.
Maf1 loss regulates spinogenesis and attenuates cognitive impairment in Alzheimer’s disease
IF=10.334，Pharmacological Research，上海交通大学附属第六医院，启动子报告基因质粒、过表达慢病毒
Imperatorin alleviates cancer cachexia and prevents muscle wasting via directly inhibiting STAT3
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Lentivirus
过表达慢病毒 干扰慢病毒
Cell Line Development
过表达细胞系构建服务 敲低细胞系构建服务 敲除细胞系构建服务 报告基因细胞株构建服务 Luciferase标记细胞株
Protein Expression
Plasmid DNA Preparation Services
过表达质粒构建 干扰质粒构建 突变/截短质粒构建 荧光素酶报告基因质粒构建
Adeno-associated Virus
过表达AAV 干扰AAV
Cell-based Assay
抗体Binding检测 蛋白/抗体药物功能检测 ADCC/P 活性检测 ADC药物CTG杀伤检测 CCK8细胞增值检测 克隆形成 细胞迁移/侵袭 细胞周期/凋亡 划痕实验
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Reporter Gene Detection Services
miRNA与靶基因调控研究 转录因子调控/promoter活性研究
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